Lipid Infiltration Measurement
Lipid Infiltration Measurement - Related Videos
10:56
A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
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Cited by 32 •
2014
A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.
08:31
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
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Cited by 2 •
2021
This protocol presents a fluorescence imaging method that uses a class of pH-sensitive lipid fluorophores to monitor lipid membrane trafficking during cell exocytosis and the endocytosis cycle.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
04:45
Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions
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Cited by 8 •
2022
Microscale thermophoresis obtains binding constants quickly at low material cost. Either labeled or label free microscale thermophoresis is commercially available; however, label free thermophoresis is not capable of the diversity of interaction measurements that can be performed using fluorescent labels. We provide a protocol for labeled thermophoresis measurements.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
FAQs
Histological assessment uses tissue sections in which lipid droplets are identified through staining and microscopy. This approach links the measured lipid signal to visible tissue architecture, allowing investigators to examine where fat is present and how it relates to structural changes. The resulting microscopic information is especially useful when cellular or localized patterns matter, rather than only an overall tissue estimate.
Magnetic resonance and computed tomography methods use differences in tissue signals to distinguish fat from other components. Their measurements therefore depend on how lipid-containing regions contrast with surrounding tissue in the selected imaging modality. This signal-based approach complements microscopy by characterizing fat within intact tissues and supports assessment of tissue composition in medical and research settings.
Comparison is useful when investigators need both microscopic localization and broader tissue characterization. Staining with microscopy can show lipid droplets in tissue sections, whereas magnetic resonance or computed tomography can separate fat from other components through signal differences. Evaluating the methods together can clarify whether an observed change reflects localized deposition, altered overall composition, or both.
Standardized lipid quantification makes measurements more comparable across patients, tissue samples, time points, or treatment groups. Consistent measurement supports interpretation of disease progression and treatment response rather than allowing differences in assessment to obscure biological change. In medicine, this reliability strengthens the use of lipid accumulation as an indicator of tissue composition, injury, and pathological progression.
A general workflow begins by selecting a measurement approach suited to the tissue and research question. Investigators may analyze stained tissue sections with microscopy or use magnetic resonance or computed tomography signals to distinguish fat from other components. The resulting lipid measurement is then interpreted alongside tissue condition, disease status, progression, or response to treatment.
Medical applications include characterizing muscle degeneration, fatty liver disease, obesity-related tissue changes, and other pathological conditions. Repeated or standardized measurements can help monitor whether lipid accumulation changes as disease progresses or treatment is introduced. The results also support studies of how abnormal fat deposition alters tissue structure and function, connecting quantitative composition with clinically relevant pathology.