Liver Necropsy
Liver Necropsy - Related Videos
21:15
Diagnostic Necropsy and Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of...
09:13
Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
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Cited by 49 •
2011
This article describes the procedures for conducting a basic postmortem examination of a mouse or rat, and the collection of basic organs, as well as more challenging sample types from for histological, microbiological, and PCR evaluation.
06:56
Rabies Necropsy Techniques in Large and Small Animals
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Cited by 2 •
2019
The goal of this protocol is to demonstrate safe necropsy techniques in small and large animals to obtain satisfactory tissue samples for rabies testing.
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
FAQs
Its diagnostic value comes from correlating gross and microscopic evidence rather than treating either view in isolation. External and internal inspection may reveal a visible lesion or altered organ characteristics, while histopathology can show corresponding cellular or tissue changes. This correlation helps investigators assess whether observed abnormalities support inflammation, fibrosis, congestion, tumors, or toxic injury.
Size, color, and texture provide complementary clues about structural change. An abnormal size may indicate that the liver has been altered globally, whereas color or texture changes may help focus attention on particular abnormalities. Lesions add location-specific information. Because these observations are descriptive rather than conclusive alone, they are strengthened by tissue examination and laboratory testing.
Representative tissue is important because a visible abnormality may not capture the full range of changes within the organ. Sampling supports histopathology, which examines microscopic changes, and can also provide material for other laboratory tests. Linking those results to the gross examination improves documentation and helps distinguish structural observations from evidence used in diagnostic conclusions.
A practical workflow begins with systematic external inspection, followed by internal examination of the liver. The examiner then records size, color, texture, and lesions, selects representative tissue, and submits it for histopathology or other laboratory testing. Keeping the observations and samples connected preserves the progression from gross appearance to microscopic or laboratory evidence.
The examination can contribute to investigation of inflammation, fibrosis, congestion, tumors, and toxic injury. These categories represent different types of pathological change, but the gross appearance alone does not establish the underlying conclusion. Microscopic findings and, when appropriate, other laboratory results add evidence that helps interpret the observed lesion or altered organ characteristics.
Beyond an individual case, documented findings can support quality assurance, medical education, and research. In clinical and pathological settings, the record provides evidence for conclusions about organ damage. In teaching, the connection between visible abnormalities and microscopic changes helps learners understand how structural findings inform medical interpretation and support broader diagnostic or research conclusions.