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JoVE Journal
Biology

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In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
 

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

Article DOI: 10.3791/3138
November 10th, 2011

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Summary November 10th, 2011

The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.

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