Microsurgical Liver Procurement
Microsurgical Liver Procurement - Related Videos
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
12:27
Technique of Porcine Liver Procurement and Orthotopic Transplantation using an Active Porto-Caval Shunt
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Cited by 15 •
2015
Experimental animal research plays a pivotal role in the development of clinical transplantation practice. The porcine orthotopic liver transplantation model (OLTx) closely resembles human conditions and is frequently used in clinically oriented research. The following protocol contains all information for a reliable porcine OLTx model using an active porto-caval-jugular shunt.
13:53
The Helsinki Rat Microsurgical Sidewall Aneurysm Model
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Cited by 21 •
2014
Microsurgical sidewall aneurysms in rats are created by end-to-side anastomosis of an aortic graft to the abdominal aorta. We present step-by-step instructions and discuss anatomical and surgical details for successful experimental saccular aneurysm creation.
05:36
Kidney Procurement in a Preclinical Large Animal Model
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2025
Here, we present a protocol that details a surgical model for kidney procurement in a preclinical swine model for subsequent machine perfusion or transplantation.
01:29
E-procurement - Buying on the Internet
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2025
Integrating information technology into business-to-business (B2B) marketing has significantly transformed how companies interact and transact. A crucial development in this domain is e-procurement, which has become standard practice for many companies. E-procurement allows buyers to access new suppliers, reduce costs, and expedite order processing and delivery. This method includes strategies such as reverse auctions, online trading exchanges, and dedicated purchasing websites, each providing...
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These structures determine whether the recovered graft can maintain blood flow and bile drainage after transplantation. Their preservation requires careful dissection and division because injury or unnecessary tissue trauma could compromise later graft use. Attention to each structure is particularly important when vessel size is small or when complex anatomy makes reconstruction necessary.
Magnification makes small vessels, ducts, and surrounding tissues easier to distinguish during dissection. Fine instruments then allow the surgeon to work around critical structures while limiting tissue trauma. This precision supports accurate handling of the hepatic artery, portal vein, hepatic veins, and bile duct, which helps preserve the anatomical elements needed for successful graft preparation.
The technique becomes especially valuable when vessels are small, anatomy is complex, or the graft will require vascular or biliary reconstruction. These circumstances increase the importance of precise anatomical control. By preserving usable structures during recovery, microsurgical handling can support graft preparation and reduce technical challenges that may otherwise affect transplantation.
After recovery, the graft is flushed and preserved under controlled conditions before transplantation. This stage follows the careful handling of its vascular and biliary structures and is intended to maintain graft quality during preparation. The preservation process therefore connects the surgical recovery phase with the graft’s subsequent use in transplantation.
Selected living-donor procedures may require this approach when the graft contains small vessels, complex anatomy, or structures that need reconstruction. Precise recovery helps protect the relevant vascular and biliary elements while limiting tissue trauma. In that setting, the technique contributes to preparing a usable graft and supports the safety and effectiveness of transplantation.
Careful recovery can support graft quality, reduce technical complications, and improve the safety and effectiveness of transplantation. These outcomes depend on protecting blood flow and bile drainage while handling the graft with minimal trauma. The value of the technique is therefore measured not only by organ recovery, but also by the condition in which the graft reaches transplantation.