Physiological Perfusion Model
Physiological Perfusion Model - Related Videos
04:44
Antegrade Perfusion Model: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
This video describes an antegrade perfusion technique of an isolated mouse heart during which a perfusate is injected through the left ventricle, and eventually, it perfuses the entire myocardium. This method can be used to isolate cardiomyocytes from murine models and generate decellularized hearts for further downstream applications.
05:30
Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model
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2025
This video shows an ex vivo technique for isolated lung perfusion. It is a tool for pulmonary research that will aid studies of various physiological and pathological mechanisms involved in lung transplantation procedures.
01:09
Clearance Models: Physiological Models
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2025
Drug clearance is a critical pharmacokinetic process involving the irreversible removal of drugs from the body through various organs over a specified time period. Physiological models are indispensable in determining organ-specific clearance, defined by the proportion of the drug eliminated per unit of time from the organ's blood volume. The organ's clearance rate depends on the blood flow to the organ and the extraction ratio (E). The extraction ratio describes the organ's proficiency in drug...
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
07:48
Glutamate and Hypoxia as a Stress Model for the Isolated Perfused Vertebrate Retina
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Cited by 16 •
2015
With this study, we introduce a standardized stress model for the isolated superfused bovine retina for future preclinical therapeutic testing. The effect of either hypoxia (pure N2) or glutamate stress (250 µM glutamate) on retinal function represented by a- and b-wave amplitudes was evaluated.
FAQs
Pressure gradients provide the driving force that moves fluid through the vascular network. Their magnitude and direction influence whether flow can reach different organs or tissue regions and support delivery of oxygen and nutrients. By controlling this driving force, researchers can examine how altered circulation affects tissue responses, waste removal, and overall organ function.
Maintaining relevant flow and pressure conditions helps the model produce responses that reflect physiological circulation rather than an artificial fluid movement pattern. This makes measurements of tissue responses, oxygen and nutrient delivery, and waste removal more useful for medical research. It also supports more meaningful evaluation of organ viability and treatment effects.
Experimental models represent circulation in a controlled physical system, allowing researchers to maintain flow and pressure while observing tissue responses. Computational models represent blood flow through modeled vascular networks and can support analysis without relying solely on a physical preparation. Both approaches help investigate circulation, but they provide different ways to evaluate function and treatment effects.
By altering or examining circulation through the vascular network, a physiological perfusion model can be used to study conditions in which blood flow is inadequate or abnormal. Researchers can assess resulting changes in tissue responses, oxygen and nutrient delivery, and waste removal. These observations help connect vascular disturbances with organ function and disease-related outcomes.
A basic workflow establishes the vascular network, applies pressure gradients to drive fluid, and maintains flow and pressure conditions that approximate normal circulation. The system then supports observation or measurement of tissue responses, delivery functions, waste removal, or organ viability. Researchers can use the controlled setup to evaluate a disease condition, drug effect, or treatment response.
These studies can provide information about tissue responses under controlled circulation conditions, including the effects of oxygen and nutrient delivery and waste removal. Depending on the research question, investigators may also assess organ viability, blood-flow-related disease effects, drug distribution, or changes produced by a therapy. The resulting measurements support comparisons between conditions or interventions.
In transplantation research, these models help evaluate organ viability under controlled flow and pressure conditions before or during investigations of preservation and use. In surgical planning, they can support examination of circulation-related factors. Their controlled structure also allows researchers to test therapies and study how interventions may affect organs or tissues without relying only on clinical observation.
Regenerative medicine can use these models to examine how engineered or repaired tissues respond when exposed to circulation-like delivery and waste-removal conditions. Maintaining relevant flow and pressure allows investigators to evaluate tissue function and treatment effects in a controlled setting. This provides a way to study whether an intervention supports viable tissue performance before broader application.