Method of Isolated <em>Ex Vivo</em> Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Kevin Nelson; Christopher Bobba; Emre Eren; Tyler Spata; Malak Tadres; Don Hayes, Jr.; Sylvester M. Black; Samir Ghadiali; Bryan A. Whitson

doi:10.3791/52309

Journal

/

Medicine

/

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated <em>Ex Vivo</em> Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

27,192 Views

•

08:59 min

•

February 25, 2015

DOI:

10.3791/52309-v

DOI:

10.3791/52309-v

•

08:59 min

February 25, 2015

•

19 Views

Kevin Nelson^1,2, Christopher Bobba^1,2, Emre Eren³, Tyler Spata⁴, Malak Tadres², Don Hayes, Jr.^5,6, Sylvester M. Black^3,7, Samir Ghadiali*^1,2,3, Bryan A. Whitson*^2,3,4

¹Department of Biomedical Engineering,Ohio State University Wexner Medical Center, ²Davis Heart & Lung Research Institute,Ohio State University Wexner Medical Center, ³The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory,Ohio State University Wexner Medical Center, ⁴Division of Cardiac Surgery, Department of Surgery,Ohio State University Wexner Medical Center, ⁵Departments of Pediatrics and Internal Medicine,Ohio State University, ⁶Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs,Nationwide Children’s Hospital, ⁷Division of Transplantation, Department of Surgery,Ohio State University Wexner Medical Center

Summary

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.