Saphenous Vein Injection
Saphenous Vein Injection - Related Videos
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
11:35
Decellularization and Recellularization Methodology for Human Saphenous Veins
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Cited by 21 •
2018
Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.
09:50
Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
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Cited by 9 •
2014
The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.
00:09
Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg
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2019
Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting.
05:15
Portal Vein Injection: A Method to Study Cancer Metastasis to the Liver
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2023
This video describes a surgical procedure to deliver mammary tumor cells to the murine liver via portal vein injection. This model permits the investigation of late stages of liver metastasis.
FAQs
The saphenous vein must be distinguished from surrounding tissues before access is attempted. Because it is a superficial vessel in the lower limb, locating and stabilizing it helps guide the needle or catheter into the vessel lumen rather than adjacent tissue. This improves delivery into venous circulation and lowers the likelihood of tissue injury or extravasation.
Its main practical value is providing an alternative route when other peripheral veins are difficult to access. The approach still requires controlled placement within a superficial vein, but its lower-limb location can make it useful when commonly selected peripheral sites are unsuitable. In medicine and research, this expands the options for intravenous administration without using the gastrointestinal route.
Incorrect placement or excessive tissue trauma can allow the administered substance to leave the vessel, a problem known as extravasation. Bleeding and local tissue injury are additional concerns. Careful anatomical identification, stabilization of the vein, and controlled administration help reduce these risks and support safer delivery into the intended venous pathway.
The procedure begins by locating the saphenous vein and stabilizing it to limit movement. A needle or catheter is then introduced into the vessel lumen, followed by administration of the selected substance. Maintaining accurate placement throughout delivery is important because it helps ensure entry into systemic blood flow and reduces the chance of extravasation or injury.
The route can deliver medications, fluids, contrast agents, and research substances, provided the intended protocol calls for venous administration. Its value lies in placing these materials directly into circulation rather than relying on gastrointestinal absorption. This makes the technique relevant to clinical drug delivery, contrast administration, and controlled experimental dosing.
Biomedical researchers may use this access route when an experiment requires controlled intravenous dosing or delivery of a research substance. It can support studies in which the administered material must enter systemic circulation through a defined venous pathway. The technique is especially relevant when other peripheral veins are difficult to access, while anatomical accuracy remains essential for interpretable dosing.