Single Cell Sorting
Single Cell Sorting - Related Videos
04:33
Isolating Single Cells from Xenopus Early Embryos and Sorting Them by Size
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2026
We describe a method for isolating single cells from Xenopus laevis early embryos and sorting them by cell size.
09:45
Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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2025
Circulating tumor cells (CTCs) are critical for the study of cancer progression and metastasis. This article presents a high-throughput, integrated protocol for CTC enrichment and single-CTC sequencing, improving capture efficiency and CTC purity while reducing contamination and sequencing costs, thereby advancing precision oncology research and clinical applications.
09:50
Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation
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Cited by 5 •
2019
Here, we describe a single cell micro-aspiration method for the separation of infected amoebae. In order to separate viral subpopulations in Vermamoeba vermiformis infected by Faustoviruses and unknown giant viruses, we developed the protocol detailed below and demonstrated its ability to separate two low-abundance novel giant viruses.
05:21
Culturing and Harvesting of HSPC Clones: A Method to Obtain the Singly-Sorted HSPC Clones
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2023
This video describes the protocol for isolating HSPCs from the bone marrow and sorting them using a fluorescent activated cell sorting technique, which is a method to obtain singly-sorted HSPCs. We culture these sorted cells to obtain HSPC clones.
13:35
Fluorescence Activated Cell Sorting of Plant Protoplasts
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Cited by 92 •
2010
A method for isolating specific cell types from plant material is demonstrated. This technique employs transgenic marker lines expressing fluorescent proteins in particular cell types, cellular dissociation and Fluorescence Activated Cell Sorting. Additionally, a growth setup is established here that facilitates treatment of Arabidopsis thaliana seedlings prior to cell sorting.
FAQs
In flow cytometric sorting, fluorescence and light scattering serve as measurable selection signals. As each cell is detected, those signals can trigger electrostatic or fluidic deflection, directing cells with selected characteristics into different collection vessels. This connection between optical measurement and physical routing allows a heterogeneous sample to be separated according to observable cellular properties.
Flow cytometric sorting uses a laser-equipped stream and electrostatic or fluidic deflection to route cells. Microfluidic and droplet-based systems are presented as alternative platforms for single-cell isolation, although the available description does not specify their separation mechanisms. Their inclusion broadens the technical options researchers can consider when selecting an approach for cell isolation.
Sorting makes cellular heterogeneity experimentally accessible by supplying separate populations rather than only a bulk measurement. This matters when a rare immune or tumor population, or a disease-associated cell state, might be masked in the overall sample. Isolated cells can then be analyzed or cultured independently, linking cellular variation to medical investigation.
A typical sorting workflow begins with a heterogeneous cell population and passes cells individually through a laser-equipped stream. Fluorescence or light-scattering signals are detected, and selected signals trigger electrostatic or fluidic deflection into separate collection vessels. The collected cells can then proceed to separate analysis, culture, or downstream experimentation.
In medicine, the method supports identification of rare immune and tumor populations and characterization of disease-associated cell states. These applications allow researchers to examine cellular groups within a mixed sample and distinguish medically relevant variation. The resulting populations provide material for studying disease biology and developing diagnostic or therapeutic strategies.
Sorted cells provide defined starting populations for genomic, transcriptomic, and functional analyses. They can also be used in downstream culture or experimentation, where investigators examine selected cells rather than an uncontrolled mixture. In medical research, this controlled input supports work on personalized diagnostics, therapies, and regenerative strategies by connecting selected cell populations with subsequent testing.