Tissue Block Culture
Tissue Block Culture - Related Videos
02:08
Assessing Muscle Contraction with Neuromuscular Blocker Treatment
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2025
This video demonstrates assessing the muscle using cultured motor neurons, Schwann cells, and myotubes, followed by treatment with a neuromuscular blocker to inhibit contraction. Color-coded time-motion graphics show a color-coded time-motion graphic. Red indicates the fastest movement, and blue indicates the slowest.
09:35
Histotypic Tissue Culture
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2023
Although two-dimensional tissue culture has been common for some time, cells behave more realistically in a three-dimensional culture, and more closely mimics native tissue. This video introduces histotypic tissue culture, where the growth and propagation of one cell line is done in an engineered three-dimensional matrix to reach high cell density. Here, we show the harvesting of cells from donor tissue, followed by cell culture on an engineered construct.
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
03:16
Intravenous Delivery of Receptor Blockers to Reduce Brain Edema in a Mouse Model
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2025
Source: Zeynalov, E. et. al., Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema. J. Vis. Exp. (2016)This video demonstrates the intravenous delivery of an arginine-vasopressin (AVP) receptor antagonist in a mouse model. Transient middle cerebral artery occlusion induces brain edema. An AVP receptor antagonist is administered through a catheter in the left jugular vein, alleviating edema.
FAQs
Nutrients and oxygen reach cells primarily by diffusion from the surrounding sterile nutrient medium. This makes the regulated environment important: temperature, gas conditions, and pH must support continued tissue function without disrupting the interactions preserved inside the block. The resulting outcome depends on maintaining conditions that allow cells to remain viable within their structural context during ex vivo study.
Keeping cells within an intact tissue piece preserves relationships among multiple cell types and the extracellular matrix. These structural and cellular interactions can influence tissue function and disease-related changes in ways that isolated cells no longer reflect. Consequently, tissue block culture can provide biologically relevant evidence when researchers need to examine responses in a more native organization.
The extracellular matrix and neighboring cells remain part of the experimental material rather than being removed before observation. Their continued presence helps maintain the tissue’s native structural context, allowing researchers to study changes across interacting components instead of focusing on one cell population alone. This is particularly relevant when investigating tissue function, disease-related changes, or repair processes.
The basic workflow places a small, intact tissue piece in sterile nutrient medium and maintains it under regulated temperature, gas, and pH conditions. These controls create the ex vivo environment needed for diffusion of nutrients and oxygen into the tissue. Maintaining sterility and stable culture conditions is therefore central to obtaining interpretable observations from the preserved tissue block.
In medical research, this approach can support studies of tissue function, disease-related changes, wound healing, and responses to drugs or other treatments. Its value is greatest when investigators need tissue-level evidence that retains interactions among cell types and the extracellular matrix. Such findings can also contribute to therapeutic development by testing biological responses in a preserved tissue context.
Tissue block culture can reveal how an intact tissue responds to a drug or other treatment while its cellular organization and extracellular matrix remain present. This produces evidence that is more biologically relevant than observations from isolated cells alone when tissue architecture affects the response. Researchers can use those results to study treatment effects and inform therapeutic development.