Dual Reporter Mesc Line
Dual Reporter Mesc Line - Related Videos
05:58
The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF
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Cited by 7 •
2009
SC1 functions through dual inhibition of Ras- GAP and ERK1. We tested the function of SC1 in supporting mouse ES cell self-renewal in the absence of LIF and showed that SC1 is able to maintain self-renewal of mouse ES cell cultures.
07:33
In vivo Dual Substrate Bioluminescent Imaging
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Cited by 20 •
2011
Herein we describe the methods to construct, visualize, and quantify the bioluminescent reactions of both firefly and renilla luciferase enzymes expressed in metastatic breast cancer cells during their growth and metastasis in vivo.
08:07
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
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2024
Here, we describe a newly developed multiplex fluorescent immunoassay that uses a dual-reporter flow cytometric system to concurrently detect two unique spike protein epitopes on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles that had been captured by angiotensin-converting enzyme-2-coupled magnetic microspheres.
02:40
Detecting SMAD Pathway Activation Using Dual Adenovirus Transduction
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2026
Source: Chen, H., et al. Live Cell Imaging of the TGF-β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System. J. Vis. Exp. (2018).This video demonstrates a method to study SMAD pathway activation in breast cancer cells using dual adenoviral infection. One virus delivers a constitutively expressed GFP gene, while the other carries an RFP reporter under a SMAD-sensitive promoter. Upon TGF-beta stimulation, SMAD proteins are activated, forming a complex that induces RFP...
05:51
Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
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2020
Here, we present a protocol describing a streamlined method for the efficient generation of plasmids expressing both the CRISPR enzyme and associated single guide RNA (sgRNAs). Co-transfection of mammalian cells with this sgRNA/CRISPR vector and a dual luciferase reporter vector that examines double-strand break repair allows evaluation of knockout efficiency.
FAQs
Each reporter is linked to a different regulatory DNA sequence, allowing its signal to reflect activation of a corresponding cell-type-associated gene program. Because the two signals can be monitored in the same culture, researchers can examine whether molecular states emerge together, sequentially, or in different cell populations. This design connects reporter dynamics with changes during neural lineage progression.
Signal separation lets investigators compare two molecular readouts within the same experimental context. Paired measurements can reveal relationships between neural progenitor emergence and neuronal development while reducing reliance on separate cultures for each state. In neuroscience experiments, this supports direct evaluation of how developmental pathways relate to one another and how experimental conditions influence cell fate.
Changes in reporter intensity or distribution can indicate that the regulatory programs controlling the selected genes have changed. During self-renewal or differentiation, comparing both signals helps identify whether cells retain an earlier state, acquire a neural progenitor-associated state, or progress toward a neuronal state. Interpretation remains tied to the genes and regulatory sequences selected for the line.
An experimental workflow can follow reporter signals over time in living cultures, then use cell sorting to separate populations according to their detectable states. Quantitative analysis of the resulting signals allows researchers to compare conditions and measure changes in neural differentiation. Live imaging adds temporal information, helping reveal how reporter patterns change during progression rather than only at a selected observation point.
These experiments are useful when a study needs to follow more than one stage or outcome of neural development in vitro. Researchers can monitor the appearance of neural progenitors and neurons, compare developmental pathways, and evaluate how experimental conditions influence cell fate. The system helps connect observed signal changes with specific steps in neural differentiation.
Data from the two reporters support visual and quantitative comparisons across experimental conditions. A study may examine whether one neural population appears alongside another, whether their signals change during lineage progression, or whether a condition shifts the observed pattern of cell fates. These outcomes provide a broader view of developmental trajectories than a single reporter measurement alone.