Electrospun Fibers
Electrospun Fibers - Related Videos
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
2015
Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
08:28
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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Cited by 6 •
2017
A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.
15:52
Postproduction Processing of Electrospun Fibres for Tissue Engineering
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Cited by 30 •
2012
Electrospun scaffolds can be processed post production for tissue engineering applications. Here we describe methods for spinning complex scaffolds (by consecutive spinning), for making thicker scaffolds (by multi-layering using heat or vapour annealing), for achieving sterility (aseptic production or sterilisation post production) and for achieving appropriate biomechanical properties.
03:55
In Vivo Single-Fiber Recording of Sciatic Nerve C-Fibers in Rats
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2025
This video demonstrates a single-fiber recording of C-fiber nerve conduction in rats. An anesthetized rat is incised to expose the sciatic nerve, which provides sensory and motor supply to the lower limbs. The individual C-fibers are then exposed. Electrical stimulation is provided using stimulus electrodes implanted in the foot, and a response is recorded from a single nerve fiber.
01:48
Classification of Skeletal Muscle Fibers
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2019
Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions. Slow-Twitch Muscle Fibers Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
FAQs
A high-voltage electric field charges the polymer solution or melt and draws it from a Taylor cone into a fine jet. Electrical forces stretch the jet as it travels toward the collector, while solvent evaporation allows the polymer to form a solid filament. This sequence determines how the material transitions from a fluid feed into a deposited fibrous architecture.
Solution properties influence how readily the charged jet stretches and solidifies, whereas collection conditions affect how fibers accumulate on the collector. Together, these factors help control fiber diameter and alignment. Adjusting them is important because the resulting architecture determines whether the scaffold provides randomly arranged or more structured physical guidance for cells and neurites.
Alignment gives the deposited filaments a preferred orientation rather than distributing them without a defined direction. In neural research, that organization can create a structured surface for examining neuronal adhesion and neurite extension. Fiber diameter also contributes to the scaffold architecture, so alignment and microscale or nanoscale dimensions provide distinct variables for studying cell behavior.
These polymer scaffolds can reproduce selected structural features of the extracellular matrix, the network surrounding cells in neural tissue. Their porous fibrous architecture offers a platform for studying how neurons interact with organized materials, including adhesion and neurite extension. This makes them relevant to investigations of nerve regeneration and the design of neural interfaces.
Production begins with a polymer solution or melt positioned for electrospinning. Applying high voltage forms a Taylor cone and launches a charged jet toward a collector. As the jet stretches, solvent evaporates, and solid fibers accumulate. The final scaffold reflects both the material feed and the collection conditions used during deposition.
Researchers may use them when a study requires a porous fibrous platform with controllable alignment or diameter. In neuroscience, applications include examining nerve regeneration, creating structured neural-interface materials, and supporting controlled delivery of bioactive molecules. The same scaffold concept therefore connects cell-material studies with engineering efforts aimed at guiding neural growth or presenting active compounds.