Mouse Neck Injection
Mouse Neck Injection - Related Videos
09:53
Neck Exam
0 Views •
2023
Source: Robert E. Sallis, MD. Kaiser Permanente, Fontana, California, USA Examination of the neck can be a challenge because of the many bones, joints, and ligaments that make up the underlying cervical spine. The cervical spine is composed of seven vertebrae stacked in gentle C-shaped curve. The anterior part of each vertebra is made up of the thick bony body, which is linked to the body above and below by intervertebral discs. These discs help provide stability and shock absorption to the...
03:30
Targeted Drug Delivery in a Mouse Model via Cisterna Magna Injection
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2025
Source: Zamboni, M., et al., Induction of Leptomeningeal Cells Modification Via Intracisternal Injection. J. Vis. Exp. (2020)This video demonstrates the procedure of delivering an inducer drug into the cisterna magna of an anesthetized mouse. The injection selectively induces fluorescent marker expression in brain surface cells accessible via cerebrospinal fluid, confirmed by fluorescence imaging.
02:36
Intramucosal Inoculation of Cancer Cells in Murine Model: A Procedure to Establish an Orthotopic Head and Neck Cancer Mouse Model
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2023
In this video, we develop an orthotopic head and neck cancer murine model by intramucosal inoculation of the squamous cell carcinoma cell line. The tumor-bearing mouse would show resemblance to clinically relevant symptoms of human disease and can thus be used to study cancer progression and discover novel therapeutic strategies.
06:53
Heterotopic Hindlimb-to-Neck Transplantation in the Mouse: A Supermicrosurgical Model of Vascularized Composite Allotransplantation
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2026
We present a reproducible mouse model of heterotopic hindlimb-to-neck vascularized composite allotransplantation. This supermicrosurgical protocol, involving 0.3-0.5 mm vessel anastomoses, integrates nylon filament guidance and venous cuffing. It provides a reliable platform for translational studies on tolerance, ischemia-reperfusion injury, and immunological mechanisms in vascularized composite allotransplantation.
03:48
Mouse Bladder Wall Injection
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Cited by 34 •
2011
Mouse bladder wall injection is a useful approach to orthotopically study bladder stem cell and cancer biology. This delicate microsurgical method can be mastered with careful technique and practice.
FAQs
The cisterna magna contains cerebrospinal fluid, allowing an injected substance to move within a fluid compartment surrounding the brain and spinal cord. This pathway can support broader exposure of central nervous system structures than delivery confined to a single local tissue site. The resulting distribution is useful when researchers need to examine neural effects across connected regions.
Accurate needle positioning helps place the material in the intended cerebrospinal fluid-filled space rather than surrounding tissues. Controlled delivery further supports consistent administration and limits unnecessary tissue injury. These factors matter because misplaced or poorly controlled injections can increase experimental variability, making it harder to distinguish biological effects from differences caused by the technique.
Reliability depends especially on the relationship between the needle path, the target space, and the control of material delivery. Careful advancement through the posterior neck helps maintain the intended route, while consistent positioning and delivery reduce differences between animals. Together, these conditions improve targeting and make experimental outcomes easier to interpret in neuroscience studies.
The approach can deliver several classes of experimental materials, including drugs, tracers, antibodies, and gene-delivery agents. Each type supports a different investigative goal, such as examining pharmacological effects, following neural pathways, evaluating molecular targets, or testing gene-related interventions. The shared advantage is access to a route associated with cerebrospinal fluid and central nervous system tissues.
A typical workflow uses a fine needle, advances it carefully through the posterior neck, and positions it in the cisterna magna before delivering the experimental material. The procedure emphasizes accurate placement and controlled administration rather than rapid delivery. Maintaining this approach helps support targeting of the intended fluid compartment while reducing avoidable tissue injury and variability.
Mouse Neck Injection is useful when investigators need to study neural pathways, neuropharmacology, or neurological disease using substances that can circulate around the brain and spinal cord. Tracers can support pathway-related studies, whereas drugs, antibodies, and gene-delivery agents enable different experimental interventions. Results can help connect administered materials with central nervous system responses.