Otic Vesicle Injection
Otic Vesicle Injection - Related Videos
03:55
Differentiating Otic Progenitor Cells Into Sensory Epithelial Cells Followed by Immunostaining
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2025
This video demonstrates the differentiation of immortalized multipotent otic progenitor (iMOP) cells into sensory epithelial cells. It outlines the steps involved in culturing iMOP cells, forming otospheres, inducing differentiation, and visualizing cellular components and differentiation markers through fluorescence microscopy to confirm successful cell differentiation.
12:17
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
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Cited by 10 •
2016
The current protocols to maintain immortalized multipotent otic progenitor (iMOP) cells and otic differentiation are described. Culture conditions and molecular markers that indicate differentiation into sensory epithelia and spiral ganglion neurons (SGN) are highlighted.
10:40
Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
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Cited by 8 •
2011
A method to deliver morpholinos directly into the zebrafish otocyst at 24hpf has been developed. Using microinjection of morpholinos into the lumen of otic vesicle and electroporation to effect penetration, we were able to bypass the effect of morpholinos on the brain and obtain effects specific to the inner ear.
05:14
Differentiating Immortalized Multipotent Otic Progenitors into Spiral Ganglion Neurons and Evaluating the Differentiation
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2025
The video demonstrates the differentiation of immortalized multipotent otic progenitors (iMOPs) into spiral ganglion neurons (SGNs) and immunofluorescence-based confirmation of the differentiation. The iMOPs are plated onto culture substrate-coated coverslips in a neuronal differentiation medium for differentiation into SGNs. The differentiated cells are labeled with antibodies specific for neuronal differentiation markers and analyzed under a microscope to confirm differentiation.
08:36
FM Dyes in Vesicle Recycling
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2023
FM dyes are a class of fluorescent molecules that has found important use in studying the vesicle recycling process. By virtue of a chemical structure, these molecules can insert themselves into the outer leaflet of phospholipid bilayer membranes. After membrane insertion, they are internalized into the cell via endocytosed vesicles, and released when these vesicles recycle back to the membrane. Since, these dyes fluoresce strongly in the hydrophobic environment within membranes and weakly in...
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The injected material enters the fluid-filled otic vesicle and can contact both the developing otic epithelium and nearby surrounding tissues. This spatial access is important because these regions contribute to sensory and neural structures associated with the inner ear. Consequently, researchers can examine how introduced signals or genetic materials influence local developmental processes rather than relying only on indirect exposure.
Cells, molecules, and genetic materials can each be introduced into the embryonic otic vesicle, allowing different experimental questions. Cells may be examined in relation to developing tissues, molecules can test developmental signals, and genetic materials can help investigate gene function. Selecting among these cargo types determines whether the study emphasizes cellular interactions, signaling, or genetic regulation.
Microscopic guidance helps the researcher position a fine glass needle within the small embryonic compartment, while controlling the injected volume limits variation between experiments. Together, these measures support more consistent delivery and help preserve the intended relationship between the introduced material and otic tissues. Consistency is especially important when comparing developmental effects or evaluating experimental treatments.
The procedure begins by preparing the embryonic otic vesicle for microscopic access, then positioning a fine glass needle under visual guidance. The researcher introduces a controlled volume containing cells, molecules, or genetic material into the fluid-filled compartment. After delivery, resulting changes can be examined in developing otic epithelium, surrounding tissues, sensory structures, or associated neural outcomes.
Researchers use this approach to study inner-ear development, neuronal patterning, and hair-cell formation in embryonic models. It provides a way to introduce defined experimental materials during development and then assess their effects on otic tissues and associated sensory structures. The method is therefore useful when the research question concerns how developmental signals or gene function shape hearing and balance systems.
Otic Vesicle Injection can be used to evaluate experimental treatments and investigate gene function in models relevant to hearing or balance disorders. Researchers may follow how introduced materials affect developing sensory structures and related auditory or vestibular function. These observations can connect developmental changes within the otic system with outcomes relevant to impaired hearing or balance.