Rectal Biopsy
Rectal Biopsy - Related Videos
03:02
Immunohistochemical Staining of S-100 Protein in Human Rectal Suction Biopsy Sections
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2025
This video demonstrates the immunohistochemical staining for S-100 protein in rectal suction biopsy sections. Antigen-retrieved sections are blocked and treated with primary, followed by secondary antibodies against S-100, a marker of Hirschsprung's disease. Chromogenic substrate is added to stain the targeted cells. Samples are dehydrated and mounted before visualizing under the microscope.
05:32
Povidone Iodine Rectal Preparation at Time of Prostate Needle Biopsy is a Simple and Reproducible Means to Reduce Risk of Procedural Infection
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Cited by 5 •
2015
Here, we present a protocol for use at the time of transrectal ultrasound guided prostate needle biopsy (TRUS PNB) that is a simple and cost-effective means to reduce infections following the procedure.
09:39
Male Rectal Exam
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2023
Source: Joseph Donroe, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT While its usefulness in cancer screening is debated, the male rectal examination remains an important part of the physical exam. The exam is indicated in selected patients with lower urinary tract symptoms, urinary and/or fecal incontinence or retention, back pain, anorectal symptoms, abdominal complaints, trauma patients, unexplained anemia, weight loss, or bone pain. There are no absolute...
05:45
Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
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Cited by 7 •
2019
This protocol describes the process of immunostaining rectal suction biopsies for calretinin, S100 protein, and protein gene product 9.5. This novel adjuvant diagnostic method for Hirschsprung's disease has preferable sensitivity and specificity rates.
01:27
Assessing Body Temperature - Rectal
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2025
Rectal temperature measurement is considered the most precise method for assessing core body temperature and typically registers higher than oral temperature. For adults, the rectal thermometer should be inserted 1 to 1.5 inches into the rectum to obtain the most accurate reading. Follow these steps for rectal temperature assessment: Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection. Step 2: Position the patient in a side-lying position to better visualize the rectal...
FAQs
Interpretation centers on the cellular organization of the sampled tissue, particularly the presence of enteric ganglion cells and nerve fibers. Enzyme-based and immunohistochemical markers add complementary evidence about abnormal innervation. Together, these observations help distinguish normal from disrupted enteric nervous system features and support evaluation of Hirschsprung disease or related neuropathies.
Including mucosa and submucosa provides tissue from the regions collected for microscopic and molecular assessment of enteric nervous system features. This sampling permits examination of ganglion cells, nerve fibers, and marker patterns within the biopsy. The resulting cellular evidence can reveal abnormalities in innervation that are relevant to intestinal motility disorders.
Microscopy shows the tissue’s cellular and structural features, while molecular and immunohistochemical analyses provide additional marker-based information. Examining enzyme or immunohistochemical patterns alongside ganglion cells and nerve fibers strengthens the assessment of abnormal innervation. This combined approach can provide more informative evidence for diagnosis and for studying enteric nervous system organization.
After a suction biopsy removes the sample, the tissue undergoes microscopic and molecular analysis. Investigators examine it for enteric ganglion cells and nerve fibers, then apply relevant enzyme or immunohistochemical markers when assessing innervation. The combined findings are interpreted to identify abnormal enteric nervous system features rather than relying on a single observation.
This procedure is used to help identify Hirschsprung disease and related enteric neuropathies. Results can contribute to diagnostic assessment and treatment decisions by showing whether sampled tissue contains abnormalities in enteric innervation. Its value comes from linking tissue-level findings with disorders that affect communication between the enteric nervous system and intestinal motility.
In neuroscience research, biopsy findings provide tissue-based evidence for examining how enteric neuronal development and signaling relate to intestinal motility. Researchers can connect ganglion-cell, nerve-fiber, and marker observations with broader questions about nervous system and gut communication. This makes the method useful not only for diagnosis, but also for investigating enteric neuropathy mechanisms.