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Bacterial Gene Expression Analysis Using Microarrays
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JoVE Journal Biologia
Bacterial Gene Expression Analysis Using Microarrays

Bacterial Gene Expression Analysis Using Microarrays

DOI:
10.3791/206-v

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29:41 min

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May 28, 2007

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1Department of Environmental Toxicology,University of California Santa Cruz – UCSC

Capitoli

  • 00:04Introduction
  • 00:24cDNA synthesis
  • 02:02cDNA purification
  • 08:56Fluorescent labeling of cDNA
  • 10:09Determining concentration of fluorescent probe with Nanodrop
  • 11:42Pre-hybridization
  • 14:46Preparation of hybridization sample
  • 19:28Hybridization
  • 24:51Washing
  • 28:02Scanning

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BacterialGene ExpressionAnalysisMicroarrays

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