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JoVE Journal Neuroscience
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Gene Lieferung in Postnatale Rattengehirn von Non-ventrikuläre Plasmid Injektion und Elektroporation

Article DOI: 10.3791/2244-v • 10:35 min • September 17th, 2010
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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Dieses Protokoll beschreibt eine nicht-virale Methode der Lieferung von Gen-Konstrukte zu einem bestimmten Bereich des Gehirns lebende Nagetier. Die Methode besteht aus Plasmid-Präparation, Mikropipette Fertigung, neugeborenen Ratten pup Chirurgie, Mikroinjektion des Konstrukts und

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Neuroscience Ausgabe 43 Gehirn Transfektion in vivo Elektroporation Mikroinjektion Ratte stereotaktische Operation
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