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Neuroscience

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JoVE Journal Neuroscience
Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth
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Organotypic Slice Culture of GFP-expressing Mouse Embryos for Real-time Imaging of Peripheral Nerve Outgrowth

Article DOI: 10.3791/2309-v • 05:43 min • March 29th, 2011
March 29th, 2011 •
1, 1
1Interdisciplinary Center for Neurosciences, Institute of Anatomy and Cell Biology, University of Heidelberg

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We present a method to prepare organotypic slices of mid-gestation mouse embryos for the cultivation and time-lapse imaging of peripheral nerve outgrowth.

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Organotypic Slice Culture GFP-expressing Mouse Embryos Real-time Imaging Peripheral Nerve Outgrowth Ex Vivo Cultivation Transgenic Mouse Line TauGFP Green Fluorescent Protein Neurons Central Nervous System Peripheral Nervous System Forelimb Innervation Pharmacological Techniques Genetic Techniques Vibratome Slice Preparation Viability Age-specific Development Spinal Nerves Spinal Cord Dorsal Root Ganglia Skeletal Tissue Muscle Tissue Embryonic Day (E) E10 To E12 Slices Tissue Culture Incubator
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