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Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

DOI:
10.3791/2706-v

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04:27 min

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May 27, 2011

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1Epigenetics and Progenitor Cells Keystone,Fox Chase Cancer Center

Capitoli

  • 00:05Titolo
  • 01:12Pupal Dissection
  • 02:19Pupal Mounting
  • 03:14Representative Images f Live SOP Cells and Differentiated External Sensory Organs
  • 03:59Conclusion

Summary

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In this video, we describe a method for live cell imaging of asymmetrically dividing sensory organ progenitor cells and epidermal cells in intact Drosophila pupae

Tags

Live-cell ImagingSensory Organ Precursor CellsDrosophila PupaeGreen Fluorescent Protein (GFP)Biological MechanismsMutantsTransgenic LinesCell Fate SpecificationAdult Peripheral Nervous System (PNS)Mechanosensory OrgansNotch-dependent Cell Fate DecisionsMicrochaetesEpitheliumPupariationNumbMitosisProtein DynamicsGFP-tagged Fusion Proteins

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