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Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis
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Investigating Outer Hair Cell Motility with a Combination of External Alternating Electrical Field Stimulation and High-speed Image Analysis

Article DOI: 10.3791/2965-v • 09:36 min • July 18th, 2011
July 18th, 2011 •
1, 1
1Division of Cell Biology and Genetics, House Ear Institute

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A reliable method to investigate outer hair cell (OHC) motile responses, including electromotility, slow motility and bending, is described. OHC motility is elicited by stimulation with an external alternating electrical field, and the method takes advantage of high-speed image recording, LED-based illumination, and last generation image analysis software.

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Outer Hair Cell Motility External Alternating Electrical Field Stimulation High-speed Image Analysis OHCs Organ Of Corti Auditory Organ Stereocilia Sound Energy Detection Transduction Cochlear Amplification Electromotility Fast Motility Motor Proteins OHC Plasma Membrane Slow Motility Cytoskeletal Reorganization OHC Bending Asymmetric Distribution Of Motor Proteins Electrical Field Stimulation Mechanical Stimuli Chemical Stimuli
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