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In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
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In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

Article DOI: 10.3791/50148-v • 10:08 min • January 5th, 2013
January 5th, 2013 •
1,2, 1, 1, 3, 4, 1
1Unit on Neural Circuits and Adaptive Behaviors, Genes Cognition and Psychosis Program, National Institute of Mental Health, 2Department of Neuroscience, Brown University - National Institutes of Health Graduate Partnership Program, 3Section on Synaptic Pharmacology, Laboratory for Integrative Neuroscience, National Institute on Alcohol Abuse and Alcoholism, 4Champalimaud Neuroscience Programme, Champalimaud Center for the Unknown

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Experience-dependent molecular changes in neurons are essential for the brain's ability to adapt in response to behavioral challenges. An in vivo two-photon imaging method is described here that allows the tracking of such molecular changes in individual cortical neurons through genetically encoded reporters.

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In Vivo Two-photon Imaging Experience-dependent Molecular Changes Cortical Neurons Brain Function Brain Disorders Monitor Gene Expression Changes Neurons Live Animal Neural Activity Repeated Observation Resolution Individual Neurons Genetically Encoded Fluorescent Reporter Track Day-to-day Experience Activity-regulated Cytoskeletal Associated Protein (Arc) Transcription Neuronal Activity Protein Product Endocytosis Of Glutamate Receptors Long-term Synaptic Plasticity Molecular Marker
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