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In vitro Cell Migration and Invasion Assays
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Biologia
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JoVE Journal Biologia
In vitro Cell Migration and Invasion Assays

In vitro Cell Migration and Invasion Assays

DOI:
10.3791/51046-v

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09:55 min

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June 01, 2014

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, , ,
1Division of Hematology/Oncology, Department of Oncology,East Carolina University

Capitoli

  • 00:02Titolo
  • 01:32Cell Culture Wound Closure Assay
  • 05:23Transwell Cell Migration Assay
  • 07:57Representative Results
  • 09:20Conclusion

Summary

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Commonly used, highly accessible methods for examining cell migration and invasion in vitro are described. The first method is the cell wound closure assay that measures cell motility. The second method is the transwell migration and invasion assay that assesses the chemotactic and invasive capacity of cells.

Tags

Cell MigrationCell InvasionWound Closure AssayTranswell AssayCell MotilityCell InvasionCancer BiologyImmunologyVascular BiologyCell BiologyBiologia dello sviluppo

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