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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
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A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Article DOI: 10.3791/51308-v • 07:59 min • March 25th, 2014
March 25th, 2014 •
1, 2, 3, 1
1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College

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Biochemical assays with recombinant human MHC II molecules can provide rapid, quantitative insights into immunogenic epitope identification, deletion, or design.  Here, a peptide-MHC II binding assay scaled to 384-well plates is described. This cost effective format should prove useful in the fields of protein deimmunization and vaccine design and development.

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High Throughput MHC II Binding Assay Quantitative Analysis Peptide Epitopes Biochemical Assays Recombinant Human MHC II Molecules Immunogenic Epitope Identification Deletion Design 384-well Format Reagent Costs Higher Throughput Experimental Design Robust Analysis Reproducible Analysis ELISA Plate Liquid Handling Robot Test Peptides Concentrations MHC II Allele Types Protein Deimmunization Vaccine Design And Development Step-by-step Instructions
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