Generation of Transgenic Hydra by Embryo Microinjection

Celina E. Juliano; Haifan Lin; Robert E. Steele

doi:10.3791/51888

Rivista

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Biologia

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Generazione di transgenici Hydra Da Embryo microiniezione

Generation of Transgenic <em>Hydra</em> by Embryo Microinjection

JoVE Journal
Biologia

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JoVE Journal Biologia

Generation of Transgenic Hydra by Embryo Microinjection

Generazione di transgenici Hydra Da Embryo microiniezione

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13,974 Views

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09:10 min

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September 11, 2014

DOI:

10.3791/51888-v

DOI:

10.3791/51888-v

•

09:10 min

September 11, 2014

•

19 Views

Celina E. Juliano¹, Haifan Lin¹, Robert E. Steele²

¹Yale Stem Cell Center and Department of Cell Biology,Yale University School of Medicine, ²Department of Biological Chemistry and the Developmental Biology Center,University of California, Irvine

Summary

Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.