Un metodo per lo screening e validazione di mutazioni resistenti contro Kinase Inhibitors
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December 07, 2014
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Emersione di resistenza genetica contro terapia con inibitori della chinasi pone sfida importante per la terapia del cancro efficace. Identificazione e caratterizzazione di mutazioni resistenti contro un farmaco di nuova concezione contribuisce a una migliore gestione clinica e di progettazione di farmaci di nuova generazione. Qui, descriviamo il nostro protocollo per lo screening e la validazione di mutazioni resistenti in vitro.
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Kesarwani, M., Huber, E., Kincaid, Z., Azam, M. A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors. J. Vis. Exp. (94), e51984, doi:10.3791/51984 (2014).
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