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Bioengineering

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JoVE Journal Bioengineering
Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
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Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke

Article DOI: 10.3791/52325-v • 09:50 min • February 12th, 2015
February 12th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Biological Systems Research, Philip Morris International R&D, Philip Morris Products S.A.

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The aim of this protocol is to expose human organotypic 3D bronchial and nasal tissue models to mainstream cigarette smoke (CS) at the air-liquid interface. The impact of CS on the tissues is then investigated using a cytochrome P450 activity assay, a cilia beating measurement, and a systems biology approach.

Tags

Repeated Exposure Impact Assessment Organotypic 3D Bronchial Tissue Culture Models Nasal Tissue Culture Models Whole Cigarette Smoke Lung Biology Lung Diseases Chronic Obstructive Pulmonary Disease Lung Cancer Underlying Mechanisms Disease Development Lung Tissues Epidemiological Studies Tissue Culture Models Submerged 2D Cell Cultures Normal Human Bronchial Epithelial Cell Cultures In Vivo Exposure Situation 3D Tissue Culture Models Air-liquid Interface Organotypic Tissue Cultures In Vitro Exposure System Mainstream CS Ciliary Beating Frequency
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