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A Whole Cell Bioreporter Approach to Assess Transport and Bioavailability of Organic Contaminants in Water Unsaturated Systems

Article DOI: 10.3791/52334-v • 13:16 min • December 24th, 2014
December 24th, 2014 •
1, 2, 1, 1
1Department of Environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 2Department of River Ecology, Helmholtz Centre for Environmental Research - UFZ

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A whole cell bioreporter assay with Burkholderia sartisoli RP037-mChe was developed to detect fractions of an organic contaminant (i.e., fluorene) available for bacterial degradation after active transport by mycelia bridging air-filled pores in a water unsaturated model system.

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Whole Cell Bioreporter Transport Of Organic Contaminants Bioavailability Water Unsaturated Systems Mycelia Mass Transfer Microbial Cell Degradation Capacity Mycelia-based Transport PAH Fluorene Model Compound Water Unsaturated Model Microcosm Bioreporter Bacterium Burkholderia Sartisoli RP037-mChe Mycelial Network Pythium Ultimum EGFP Expression Confocal Laser Scanning Microscopy (CLSM)
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