A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits

Tatsuhiko Goto; Atsushi Toyoda

doi:10.3791/52973

Un modello murino di subcronica e Mild Sconfitta sociale stress per la comprensione deficit compo...

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A Mouse Model of Subchronic and Mild Social Defeat Stress for Understanding Stress-induced Behavioral and Physiological Deficits

Un modello murino di subcronica e Mild Sconfitta sociale stress per la comprensione deficit comportamentali e fisiologiche allo stress indotto

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08:10 min

November 24, 2015

DOI: 10.3791/52973-v

Tatsuhiko Goto^1,2, Atsushi Toyoda^1,2,3

¹College of Agriculture,Ibaraki University, ²Cooperation between Agriculture and Medical Science,Ibaraki University, ³United Graduate School of Agricultural Science,Tokyo University of Agriculture and Technology

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Overview

This article describes methods for developing a mouse model of subchronic and mild social defeat stress (S-C-S-D-S) to study the mechanisms of stress-induced symptoms. The model is used to investigate features of depression, including hyperphagia and polydipsia-like symptoms, following increased body weight.

Key Study Components

Area of Science

Neuroscience
Behavioral Science
Psychiatry

Background

Understanding stress-induced symptoms is crucial for developing effective treatments for depression.
Animal models are essential for studying the underlying mechanisms of mental health disorders.
Social defeat stress is a recognized method to induce depressive-like symptoms in rodents.
Identifying biomarkers for depression can aid in the diagnosis and treatment of the disorder.

Purpose of Study

To establish a reliable mouse model of S-C-S-D-S.
To investigate the behavioral and physiological changes associated with stress-induced depression.
To explore potential biomarkers for depression in humans.

Methods Used

Habituation of male black six and ICR mice to the environment.
Screening and selection of aggressive ICR mice.
Repeated exposure of S-C-S-D-S mice to black six mice over several days.
Measurement of social behavior scores, body weight, food intake, and water intake.

Main Results

Significant changes in social behavior scores were observed in S-C-S-D-S mice.
Increased body weight was noted following exposure to social defeat stress.
Hyperphagia and polydipsia-like symptoms were recorded in the model.
The findings support the use of this model for studying depression-related mechanisms.

Conclusions

The S-C-S-D-S mouse model effectively mimics stress-induced depressive symptoms.
This model can be utilized to identify biomarkers for depression.
Further research may lead to better understanding and treatment of depression in humans.

Frequently Asked Questions

What is the significance of the S-C-S-D-S model?

The S-C-S-D-S model helps researchers study the mechanisms of stress-induced depression and identify potential biomarkers.

How are the mice habituated to the environment?

Mice are gradually acclimated to the testing environment to reduce stress before experiments.

What behavioral changes are measured in this study?

Social behavior scores, body weight, food intake, and water intake are measured to assess stress effects.

Why is it important to study animal models of depression?

Animal models allow researchers to explore the biological mechanisms of depression and test new treatments.

What are hyperphagia and polydipsia?

Hyperphagia refers to increased food intake, while polydipsia refers to excessive drinking, both of which can be symptoms of depression.

Qui, metodi per sviluppare un modello di topo di subcronica e mite lo stress sconfitta sociale sono descritti e usati per studiare le caratteristiche patogene di depressione, tra cui hyperphagia- e sintomi simil-polidipsia seguenti aumento di peso corporeo.

L'obiettivo generale di questa procedura è quello di stabilire un modello murino di stress da sconfitta sociale subcronico e lieve o S-C-S-D-S per studiare i meccanismi dei sintomi indotti dallo stress. Ciò si ottiene abituando prima i singoli topi maschi neri e i topi ICR all'ambiente. Il secondo passo consiste nello screening e nella selezione di topi ICR aggressivi.

Successivamente, i topi S-C-S-D-S vengono esposti individualmente a sei topi neri, ripetutamente per diversi giorni. In definitiva, i punteggi del comportamento sociale misurati, il peso corporeo, l'assunzione di cibo e l'assunzione di acqua rivelano tutti i cambiamenti indotti dallo stress nel modello murino S-C-S-D-S. Al fine di trovare biomarcatori e sviluppare tic oggettivi per i vari tipi di depressione nell'uomo, è importante stabilire diversi modelli animali di deterioramento.

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