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Immunology and Infection

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T-celler Capture bakterier ved Transinfection fra dendritiske celler

Article DOI: 10.3791/52976-v • 11:39 min • January 13th, 2016
January 13th, 2016 •
1, 1,2, 1,2, 1,2, 1
1Cellular and Molecular Biology, Spanish National Research Council (CSIC), Spanish National Biotechnology Centre (CNB-CSIC), 2Hospital de Santa Cristina, Healthcare Research Institute Princesa Hospital (IIS-Princesa)

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Her en protokol præsenteres til at måle bakteriel fange af CD4 + T-celler, som opstår i løbet af antigen præsentation via transinfection fra præ-inficerede dendritiske celler (DC). Vi viser, hvordan du udfører de nødvendige skridt: isolering af primærelementer, infektion af DC / T-celle-konjugat dannelse, og måling af bakteriel T-celle transfektion.

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Immunologi transinfection antigenpræsentation T-celler dendritiske celler bakterier gentamicin overlevelse assay
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