Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Pamela N. Peters; Elizabeth M. Schryver; Ernst Lengyel; Hilary Kenny

doi:10.3791/53541

Rivista

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Medicina

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Modellazione Piazza precoci di cancro ovarico Diffusione in una cultura Organotipica della Cavità peritoneale umano

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

JoVE Journal
Medicina

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JoVE Journal Medicina

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Modellazione Piazza precoci di cancro ovarico Diffusione in una cultura Organotipica della Cavità peritoneale umano

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26,535 Views

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09:00 min

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December 31, 2015

DOI:

10.3791/53541-v

DOI:

10.3791/53541-v

•

09:00 min

December 31, 2015

•

4 Views

Pamela N. Peters¹, Elizabeth M. Schryver¹, Ernst Lengyel¹, Hilary Kenny¹

¹Department of Obstetrics and Gynecology, Section of Gynecologic Oncology,University of Chicago

Summary

Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.