Una nuova strategia che unisce Array-CGH, sequenziamento dell'intero-esoma e nell'Utero elettroporazione in roditori per identificare geni causativi per le malformazioni del cervello
8,642 Views
•
08:22 min
•
December 01, 2017
DOI:
Summary
Automatically generated
Eterotopia nodulare periventricolare (PNH) è la forma più comune di malformazione dello sviluppo corticale (MCD) nell'età adulta, ma la base genetica rimane sconosciuta nei casi più sporadici. Recentemente abbiamo sviluppato una strategia per identificare nuovi geni candidati per MCDs e confermare direttamente loro ruolo causativo in vivo.
Video correlati
09:17
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
Video correlati
9554 Views
08:24
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Video correlati
17765 Views
13:12
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
Video correlati
19876 Views
09:30
Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
Video correlati
43035 Views
09:39
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
Video correlati
9868 Views
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
Video correlati
13904 Views
04:01
Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures
Video correlati
1687 Views
10:29
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Video correlati
11750 Views
08:22
A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
Video correlati
8.6K Views
10:00
In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
Video correlati
9.5K Views
Read Article
Cite this Article
Conti, V., Carabalona, A., Pallesi-Pocachard, E., Leventer, R. J., Schaller, F., Parrini, E., Deparis, A. A., Watrin, F., Buhler, E., Novara, F., Lise, S., Pagnamenta, A. T., Kini, U., Taylor, J. C., Zuffardi, O., Represa, A., Keays, D. A., Guerrini, R., Falace, A., Cardoso, C. A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations. J. Vis. Exp. (130), e53570, doi:10.3791/53570 (2017).
Copy