Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Salvatore Coniglio; Ian Miller; Marc Symons; Jeffrey E. Segall

doi:10.3791/53990

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Coculture saggi per studiare macrofagi e microglia stimolazione del glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

JoVE Journal
Ricerca sul cancro

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JoVE Journal Ricerca sul cancro

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture saggi per studiare macrofagi e microglia stimolazione del glioblastoma Invasion

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13,893 Views

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09:23 min

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October 20, 2016

DOI:

10.3791/53990-v

DOI:

10.3791/53990-v

•

09:23 min

October 20, 2016

•

5 Views

Salvatore Coniglio¹, Ian Miller², Marc Symons³, Jeffrey E. Segall⁴

¹New Jersey Center for Science, Technology and Mathematics,Kean University, ²Department of Physiology and Medical Physics,Royal College of Surgeons in Ireland, ³The Feinstein Institute for Medical Research at North Shore-LIJ, ⁴Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center,Albert Einstein College of Medicine

Trascrizione

Summary

Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.