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Biochemistry

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JoVE Journal Biochemistry
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Article DOI: 10.3791/54527-v • 08:32 min • October 23rd, 2016
October 23rd, 2016 •
1, 1,2, 1,2, 1
1Department of Molecular, Cellular, and Developmental Biology, University of Michigan, 2Howard Hughes Medical Institute, University of Michigan

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This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

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In Vivo In Vitro PH-dependent Activity Escherichia Coli Chaperone HdeB Acidic PH Conditions Acid Activated Chaperones Protein Unfolding Protein Degradation Enteric Bacteria Gut Colonization Acid Protective Chaperones HDA Stress Conditions Thermally Unfolding Porcine Mitochondrial Malate Dehydrogenase Aggregation Buffer C
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