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Journal / Developmental Biology / Organ Culture and Whole Mount Immunofluorescence…
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Developmental Biology

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JoVE Journal Developmental Biology
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
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Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

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We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

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Organ Culture Whole Mount Immunofluorescence Staining Mouse Wolffian Ducts Developmental Biology Organ Shape Organ Structure Organ Function In Vivo Systems Cell Culture Studies Niche Factors Post-conception Pregnant Mouse Surgical Procedure Uterus HBSS Solution
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