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In Ovo Electroporation in de Kip Auditory Brainstem

Article DOI: 10.3791/55628-v • 10:14 min • June 9th, 2017
June 9th, 2017 •
1, 1, 1,2,3
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders, Northwestern University, 2Knowles Hearing Research Center, Northwestern University, 3Department of Neurobiology, Northwestern University

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Auditieve hersenstam neuronen van avians en zoogdieren zijn gespecialiseerd in snelle neurale codering, een fundamenteel proces voor normale gehoorfuncties. Deze neuronen ontstaan ​​uit duidelijke precursoren van embryonale hindbrain. We presenteren technieken die elektroporatie gebruiken om genen uit te drukken in het achterhoofd van kippenembryo's om de genfunctie tijdens de auditieve ontwikkeling te bestuderen.

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Ontwikkelingsbiologie Auditory brainstem avian development, Nucleus laminaris nucleus magnocellularis plasmide
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