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Biochemistry

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Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Article DOI: 10.3791/56250-v • 12:47 min • March 12th, 2018
March 12th, 2018 •
*1, *1, 2, 3, 3, 1, 4
1School of Public Health, Nanjing Medical University, 2School of Pharmacy, Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University
* These authors contributed equally

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This protocol describes a general strategy to regenerate commercial arrayed gold microelectrodes equipped for a label-free cell analyzer aimed at saving on the high running costs ofmicrochip-based assays. The regeneration process includes trypsin digestion, rinsing with ethanol and water, and a spinning step, which enables repeated usage of microchips.

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Regeneration Arrayed Gold Microelectrodes Real-Time Cell Analyzer RTCA Disposable Gold-based Chips Regeneration Procedure Lab Reagents Low Toxicity Incubation Proliferation A549 Cells Cell-culture Dish Cell Medium Electronic Plate L8 Equilibration CO2 Incubator Real-Time Cell Analyzer Operation Program Experiment Pattern Setup Assay Layout Page Experimental Steps
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