Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Subcellular Fractionation for ERK Activation Upon …
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biology
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
Journal (Biology)
Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Journal (Biology)
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
 

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Article DOI: 10.3791/56496-v • 07:55 min • September 25th, 2017
September 25th, 2017 •
1, 1, 1, 1
1Leonard Davis School of Gerontology, University of Southern California

Capitoli

Riepilogo
Automatic Translation

This protocol describes how to stimulate cells with mitochondrial-derived peptides and assess the signaling cascade and localization of phospho-proteins.

Tags

Subcellular Fractionation ERK Activation Mitochondrial-derived Peptides Downstream Signaling Cascade Signaling Pathways Mitochondrial-derived Peptide Field Subscale Kersher Method Humanin-mediated ERK Signaling SH-SY5Y Cells HEK293 Cells S14G Humanin Peptides Distilled Water Serum-containing Medium Serum-free Medium Working Concentration Cell Suspension
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter