Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Optimal Preparation of Formalin Fixed Samples for …
JoVE Journal
Biochemistry

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Journal (Biochemistry)
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Journal (Biochemistry)
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
 

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Article DOI: 10.3791/56778-v • 08:16 min • January 16th, 2018
January 16th, 2018 •
1,2, 2, 3, 4, 1, 4, 3, 1
1Mass Spectrometry Core Facility, University of Sydney, 2Proteomics Core Facility, University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney

Capitoli

Riepilogo
Automatic Translation

This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.

Tags

Formalin Fixed Samples Peptide-based Matrix Assisted Laser Desorption/ionization Mass Spectrometry Imaging Workflows Tissue Sample Preparation Peptide Mass Spectrometry Imaging Nitrocellulose Coated Slides Vapor Chambers Custom Vapor Chambers Thick Blotting Paper Plastic Petri Dish FFPE Tissue Float Mount Xylene
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter