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Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

Article DOI: 10.3791/57617-v • 06:40 min • May 22nd, 2018
May 22nd, 2018 •
1,2, 1,2, 2,3, 1, 1, 1, 1,2, 1,2, 2,3, 1,2
1Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, 2DZHK (German Center for Cardiovascular Research), 3Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

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Here, we present a protocol for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cAMP dynamics using an ex vivo Langendorff setup.

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Intracardiac Neurons Cardiac Electrophysiology Arrhythmogenesis Ex Vivo Langendorff System Autonomic Nervous System CAMP Dynamics Decentralized Hearts Structural Heart Disease Water Bath Perfusion Solution Oxygen And Carbon Dioxide Mixture Pump Rate Cannula Apparatus Targeted Perfusion Pressure Recording Electrophysiology Catheter Platinum Electrodes Electrode Surface And Spacing Data Recording And Stimulation Digital Stimulus Generator
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