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Journal / Biochemistry / Dynamisk proteom og miRNA analyse af Polysomes…
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Biochemistry

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JoVE Journal Biochemistry
Dynamisk proteom og miRNA analyse af Polysomes fra isolerede mus hjertet efter Langendorff Perfusion
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Dynamisk proteom og miRNA analyse af Polysomes fra isolerede mus hjertet efter Langendorff Perfusion

Article DOI: 10.3791/58079-v • 07:54 min • August 29th, 2018
August 29th, 2018 •
1,2,3, 1, 1, 1, 1,2, 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences

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Her præsenterer vi en protokol for at udføre polysome profilering på isolerede perfunderet mus hjerte. Vi beskriver metoder til hjertet perfusion, polysome profilering og analyse af polysome fraktioner mRNAs, miRNAs og polysome proteomet.

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Biokemi spørgsmålet 138 iskæmi og reperfusion dynamisk proteomet polysome profilering miRNA mRNA nyligt syntetiseret proteiner protein oversættelse saccharose tæthed gradient sedimentering isolerede mus hjerte perfusion Langendorff perfusion Proteomics.
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