Identificazione dei Mouse e dei repertori di anticorpo umano mediante sequenziamento di nuova generazione
14K visualizzazioni
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Citato da 4
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08:51 min.
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15 marzo 2019
DOI :10.3791/58804-v
15 marzo 2019
•14K visualizzazioni
Lin Sun1 , Naoko Kono2 , Hiroyuki Toh3 , Hanbing Xue1 , Kaori Sano4,5 , Tadaki Suzuki4 , Akira Ainai4 , Yasuko Orba6 , Junya Yamagishi7,8 , Hideki Hasegawa4,5 , Yoshimasa Takahashi9 , Shigeyuki Itamura2 , Kazuo Ohnishi9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba
Qui, descriviamo i protocolli per l'analisi e la visualizzazione della struttura e costituzione di repertori di anticorpo intero. Ciò comporta l'acquisizione di vaste sequenze di RNA di anticorpo usando il sequenziamento di nuova generazione.
Chapters in this video
0:04
Title
1:23
Nucleic Acid Isolation from Immune Cells and Tissues
3:04
cDNA Synthesis and PCR Amplification
4:58
NGS Sequencing of Libraries
5:33
Results: Identification of Mouse and Human Antibody Repertoires
8:11
Conclusion
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