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A Middle Cerebral Artery Occlusion Technique for Inducing Post-stroke Depression in Rats

Article DOI: 10.3791/58875-v • 04:38 min • May 22nd, 2019
May 22nd, 2019 •
*1, *2, 3, 1, 1, 1, 1, 1, 1, 1
1Department of Anesthesiology and Critical Care, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, 2Department of Neurosurgery, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, 3Department of Biophysics and Biochemistry, Faculty of Biology, Ecology, and Medicine, Oles Honchar Dnipro National University
* These authors contributed equally

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Here we present a protocol to induce post-stroke depression in rats by occluding the middle cerebral artery via the internal carotid artery. We use the Porsolt forced swim test and the sucrose preference test to confirm and evaluate induced depressive moods.

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Middle Cerebral Artery Occlusion Technique Post-stroke Depression Rats Mechanism Efficient Therapy Weight Changes Brain Edema Infarct Volume Procedure-related Deaths Tail Pinch Response Core Body Temperature Ointment Shaving Hair Disinfecting Skin Sterile Surgical Drape Neurological Severity Score Gait Response Infarct Volume Measurement Brain Slices
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