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Journal / Biochemistry / 放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析…
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Biochemistry

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JoVE Journal Biochemistry
放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析
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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析

Article DOI: 10.3791/58952-v • 08:59 min • February 12th, 2019
February 12th, 2019 •
1,2, 1, 1, 1, 1, 1
1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

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在这里, 我们描述了一种一般脉冲追逐方法的协议, 该方法允许在活细胞中对蛋白质的折叠、迁移和降解进行动力学分析。

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生物化学 第144期 蛋白质折叠 放射性标记 sds-page 免疫沉淀 脉冲追逐 构象分析 动力学
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