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JoVE Journal Genetics
Kromatin Immunutfelling analysen bruke Micrococcal Nukleaser i pattedyrceller
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Journal (Genetics)
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Kromatin Immunutfelling analysen bruke Micrococcal Nukleaser i pattedyrceller

Article DOI: 10.3791/59375-v • 11:42 min • May 10th, 2019
May 10th, 2019 •
1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope

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Kromatin immunutfelling (ChIP) er et kraftig verktøy for å forstå den molekylære mekanismer av gen regulering. Imidlertid innebærer metoden vanskeligheter med å få reproduserbar kromatin fragmentering av mekanisk klipping. Her gir vi en forbedret protokoll for en ChIP-analysen ved hjelp av enzymatisk fordøyelsen.

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Genetikk gen regulering histone modifikasjon kromatin immunutfelling micrococcal nuklease RNA polymerase II trimethylated histone H3 lysin 4
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