Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Mafalda Laranjo; Maria Jo&#227;o Carvalho; Beatriz Serambeque; Andr&#233; Alves; Carlos Miguel Marto; Isabel Silva; Artur Paiva; Maria Filomena Botelho

doi:10.3791/60022

Rivista

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Ricerca sul cancro

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Ottenere le sfere delle cellule staminali tumorali da tumori ginecologici e tumori del cancro

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

JoVE Journal
Ricerca sul cancro

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JoVE Journal Ricerca sul cancro

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Ottenere le sfere delle cellule staminali tumorali da tumori ginecologici e tumori del cancro

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10,114 Views

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07:01 min

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March 01, 2020

DOI:

10.3791/60022-v

DOI:

10.3791/60022-v

•

07:01 min

March 01, 2020

•

10110 Views

Mafalda Laranjo*^1,2,3,4, Maria João Carvalho*^1,2,3,4,5,6, Beatriz Serambeque^1,2,3, André Alves^2,7, Carlos Miguel Marto^1,2,3,4,8, Isabel Silva⁹, Artur Paiva^3,9,10, Maria Filomena Botelho^1,2,3,4,8

¹Institute of Biophysics, Faculty of Medicine,University of Coimbra ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra ⁴Clinical Academic Center of Coimbra (CACC)⁵Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra ⁶Gynecology A Service,Coimbra Hospital and Universitary Center ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra ⁸Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra ⁹Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences

Summary

Lo scopo di questa metodologia è quello di identificare le cellule staminali tumorali (CSC) nelle linee cellulari del cancro e nei campioni tumorali umani primari con il protocollo di formazione della sfera, in modo robusto, utilizzando saggi funzionali e caratterizzazione fenotipica con citometria di flusso e occidentale Macchia.