Ottenere le sfere delle cellule staminali tumorali da tumori ginecologici e tumori del cancro

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Citato da 9

07:01 min.

1 marzo 2020

10.3791/60022-v

1 marzo 2020

9.7K visualizzazioni

Mafalda Laranjo^*¹^,²^,³^,⁴ , Maria João Carvalho^*¹^,²^,³^,⁴^,⁵^,⁶ , Beatriz Serambeque¹^,²^,³ , André Alves²^,⁷ , Carlos Miguel Marto¹^,²^,³^,⁴^,⁸ , Isabel Silva⁹ , Artur Paiva³^,⁹^,¹⁰ , Maria Filomena Botelho¹^,²^,³^,⁴^,⁸

¹Institute of Biophysics, Faculty of Medicine, University of Coimbra, ²Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, ³CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, ⁴Clinical Academic Center of Coimbra (CACC), ⁵Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, ⁶Gynecology A Service, Coimbra Hospital and Universitary Center, ⁷Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, ⁸Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, ⁹Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, ¹⁰Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences

^* These authors contributed equally

Lo scopo di questa metodologia è quello di identificare le cellule staminali tumorali (CSC) nelle linee cellulari del cancro e nei campioni tumorali umani primari con il protocollo di formazione della sfera, in modo robusto, utilizzando saggi funzionali e caratterizzazione fenotipica con citometria di flusso e occidentale Macchia.