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JoVE Journal Genetics
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Article DOI: 10.3791/60085-v • 09:04 min • September 25th, 2019
September 25th, 2019 •
*1, *1, 1,2,3, 1,2,3
1Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, University of Pennsylvania
* These authors contributed equally

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This protocol provides a method to facilitate the generation of defined heterozygous or homozygous nucleotide changes using CRISPR-CAS9 in human pluripotent stem cells.

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CRISPR-Cas9 Genomic Modifications Human Pluripotent Stem Cells Gene Function Disease IPS Cell Lines Differentiation Efficiency Phenotypes Functional Assay Isogenic Lines Heterozygous Mutations Indel Mutations Repair Templates Colony Picking Stem Cell Culture Transfection Master Mix
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