Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Screening for Thermotoga maritima Membrane-Bound…
JoVE Journal
Biochemistry

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biochemistry
Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Journal (Biochemistry)
Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics
Journal (Biochemistry)
Preparation of Keratin Hydrolysate from Chicken Feathers and Its Application in Cosmetics
 

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

Article DOI: 10.3791/60619-v • 09:11 min • November 23rd, 2019
November 23rd, 2019 •
*1, *2, 2, 2, 2, 2, 2, 1,3
1Research Program in Molecular and Integrative Biosciences, University of Helsinki, 2Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, 3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Here we present a screening method for membrane-bound pyrophosphatase (from Thermotoga maritima) inhibitors based on the molybdenum blue reaction in a 96 well plate format.

Tags

Thermotoga Maritima Membrane-bound Pyrophosphatase Inhibitors Compounds Parasitic Diseases Treatment Protocol Reactivation Buffer Solution M-E-S Glycerol D-T-T D-D-M Reaction Mixer Tris Hydrochloride Magnesium Chloride Potassium Chloride Sodium Chloride Liposomes Enzyme Reactivation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter