Rivista
/
Ricerca sul cancro
/
从细胞和组织样本中分离和功能评估人类乳腺癌干细胞
JoVE Journal
Ricerca sul cancro
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Ricerca sul cancro
Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
Please note that all translations are automatically generated. Click here for the English version.

从细胞和组织样本中分离和功能评估人类乳腺癌干细胞

DOI:
10.3791/61775-v

•

07:03 min

•

October 02, 2020

•

, , , ,
1London Regional Cancer Program, 2Department of Anatomy & Cell Biology,Western University, 3Department of Oncology,Western University, 4Lawson Health Research Institute

Capitoli

  • 00:05Introduction
  • 00:38Breast Cancer Cell Culture Single Cell Suspension Generation
  • 01:22Tissue Sample Single Cell Suspension Generation
  • 02:45Colony Forming Assay
  • 03:54Mammosphere Assay and 3D Culture Model
  • 05:09Results: Representative BCSC Identification and Functional Evaluation
  • 06:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

该实验方案描述了从乳腺癌细胞和组织样品中分离BCSC以及可用于评估BCSC表型和功能的 体外 和 体内 测定。

Tags

Breast Cancer Stem CellsCell IsolationSingle Cell SuspensionALDH AssayColony Forming AssayFunctional CharacterizationTumorigenicityMetastasis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

巨蟹座的建立源于人类传统的骨肉瘤细胞培养

利用功能基因组筛选识别在肿瘤细胞球体培养潜在的新药物靶标

合成与阿司匹林富马酸酯前药的表征抑制NFκB活动和乳腺癌干细胞

在斑马鱼胚胎人乳腺癌细胞的侵袭行为

骨髓间充质干细胞在浆组织中的分离及与癌细胞联合培养研究其相互作用

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

从妇科和乳腺癌肿瘤获得癌症干细胞球

从3维球体培养中分离出类似干细胞的细胞

使用 CRISPR 介质基础编辑器对 BRCA1 变体进行功能评估

使用微生理系统模拟人体乳房组织中的乳腺癌

Read Article