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Journal / Neuroscience / Långsiktig, serumfri odling av organotypiska…
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Neuroscience

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JoVE Journal Neuroscience
Långsiktig, serumfri odling av organotypiska musreta explanter med intakt retinalpigmentepitetel
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Långsiktig, serumfri odling av organotypiska musreta explanter med intakt retinalpigmentepitetel

Article DOI: 10.3791/61868-v • 05:52 min • November 25th, 2020
November 25th, 2020 •
*1, *1, 1, 1, 1, 1
1Institute for Ophthalmic Research, University of Tübingen
* These authors contributed equally

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Protokollet beskriver organotypiska explanter av mus neuronäthinnan, odlas tillsammans med dess retinal pigment epitel (RPE), i R16 definierade medium, fria från serum och antibiotika. Denna metod är relativt enkel att utföra, billigare och tidskrävande jämfört med in vivo-experiment, och kan anpassas till många experimentella applikationer.

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Neurovetenskap Utgåva 165 neuroretina RPE organotypiska explantkulturer serumfritt medium mus organkultur
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