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Herstellung von Ratten-Skelettmuskelhomogenaten für Nitrat- und Nitritmessungen
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Herstellung von Ratten-Skelettmuskelhomogenaten für Nitrat- und Nitritmessungen

Article DOI: 10.3791/62427-v • 07:19 min • July 29th, 2021
July 29th, 2021 •
*1, *1, 2, 2, 2, 1, 1
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus, University of Exeter
* These authors contributed equally

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Wir präsentieren Protokolle für drei verschiedene Methoden zur Homogenisierung von vier verschiedenen Muskelgruppen von Ratten-Skelettmuskelgewebe, um die Nitrat- und Nitritwerte zu messen und zu vergleichen. Darüber hinaus vergleichen wir verschiedene Probengewichte, um zu untersuchen, ob die Gewebeprobengröße die Ergebnisse der Homogenisierung beeinflusst.

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Biologie Ausgabe 173 Nitrat Nitrit Stickstoffmonoxid Skelettmuskulatur Gewebehomogenisierung
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