Imaging e quantificazione di dendriti neuronali intatti tramite CLARITY Tissue Clearing
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April 20, 2021
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Summary
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La morfologia dendritica neuronale è spesso alla base della funzione. In effetti, molti processi patologici che influenzano lo sviluppo dei neuroni si manifestano con un fenotipo morfologico. Questo protocollo descrive un metodo semplice e potente per analizzare i pergole dendritiche intatte e le loro spine associate.
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Pekarek, B. T., Hunt, P. J., Belfort, B. D. W., Liu, G., Arenkiel, B. R. Imaging and Quantification of Intact Neuronal Dendrites via CLARITY Tissue Clearing. J. Vis. Exp. (170), e62532, doi:10.3791/62532 (2021).
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